Combination of a high-carbohydrate diet and streptozotoc in for modeling type 2 diabetes in Wistar rats

Relevance. To conduct a preclinical evaluation of the effectiveness antidiabetic drugs, models simulating pathogenesis and main manifestations diabetes mellitus (DM) in humans are needed. The streptozotocin (STZ) model, which has received most widespread use experiment, does not allow reproducing stepwise multifactorial development type 2 diabetes. Goal. develop model using high-carbohydrate diet combination with subthreshold dose STZ Wistar rats, characterized by hyperglycemia insulin resistance. Methods. animals control group ( n = 20) water as drink, experimental 10 % solution fructose. After 14 days, from each were injected at 35 mg/kg. blood glucose level was determined weekly. assess resistance, oral tolerance test performed before after administration STZ. Results. It found that keeping rats on for two weeks leads to violation tolerance, indicates introduction mg/kg standard causes an increase glycemic drop 13.2 mmol/l, while same against background 22.9 mmol/l increases Conclusion. synergism low doses makes it possible obtain reproduces only basal hyperglycemia, but also impaired more fully corresponds process developing humans.